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GenDEPOT公司 Western Blot预制缓冲液及相关产品目录

用于蛋白质印迹的预制缓冲液产品

1. 样品裂解

类别	产品名称	货号	规格
裂解缓冲液	RIPA Cell Lysis Buffer (1X) with EDTA	R4100-010	100 ml
裂解缓冲液	RIPA II Cell Lysis Buffer with Triton (1X), without EDTA	R4200-010	100 ml
蛋白酶/磷酸酶抑制剂混合物溶液	Xpert Protease Inhibitor Cocktail Solution (100X)	P3100-001	1 ml
	Xpert Phosphatase Inhibitor Cocktail Solution (100X)	P3200-001	1 ml
	Xpert Duo Inhibitor Cocktail Solution (100X)	P3300-001	1 ml
蛋白琼脂糖	Puredown Protein A-Agarose, BSA-Binding Site Blocked	P9201-015	1.5 ml
	Puredown Protein G-Agarose, BSA-Binding Site Blocked	P9202-015	1.5 ml
	Puredown Protein A/G-Agarose, BSA-Binding Site Blocked	P9203-015	1.5 ml
蛋白质定量	Bradford (Coomassie) Protein Assay Plus Kit	P7200-050	Kit
蛋白质定量	Bicinchoninic Acid (BCA) Protein Assay Kit	P8100-050	Kit
蛋白质样品缓冲液	Laemmli Sample Buffer (4X), Reducing	L1100-001	10 ml
	Laemmli Sample Buffer (4X), Non-Reducing	L1200-001	10 ml
	Native Sample Buffer (4X)	N2300-002	20 ml

2. SDS-PAGE

类别	产品名称	货号	规格
SDS-PAGE凝胶	Acrylamide : bis 30%, 29:1 Solution	A0418-050	495 ml
	Acrylamide : bis 40%, 29:1 Solution	A0421-050	495 ml
	1.5M Tris-HCl Buffer (pH 8.8)	T8101-050	500 ml
	0.5M Tris-HCl Buffer (pH 6.8)	T8102-050	500 ml
	10％ SDS Solution	S0792-050	500 ml
	20％ SDS Solution	S0793-050	500 ml
	Ammonium Persulfate (APS) Tablets, 150 mg	A7503-020	20 Tablets
	TEMED	T0512-002	25 ml
运行染色液	Tris, Ultra Pure	T9200-100	1 Kg
	Glycine, 99％	G0610-100	1 Kg
	SDS, 99％ Ultra Pure	S0800-100	1 Kg
	10X Tris-Glycine SDS buffer (Running buffer)	T8053-050	500 ml
蛋白质标志物	Prosi Prestained Protein Marker	P8500-040	2 X 200 μl
	Xpert Prestained Protein Marker	P8502-050	2 X 250 μl
	Xpert 2 Prestained Protein Marker	P8503-050	2 X 250 μl
凝胶染色	ProsiBlue Gel Staining Solution	P7300-050	500 ml

3. (Wet) Transfer

类别	产品名称	货号	规格
转印膜	West-Q PVDF Membrane, Roll, 0.22 um, 270mm x 3M	W7031-270	Roll
	West-Q PVDF Membrane, Roll, 0.45 um, 270mm x 3M	W7032-270	Roll
	NitroPure Nitrocellulose Transfer Membrane,Roll, 0.22 um , 300mm x 3M	LC7033-300	Roll
	NitroPure Nitrocellulose Transfer Membrane,Roll, 0.45 um , 300mm x 3M	LC7034-300	Roll
转印缓冲液	10X Tris-Glycine Native Buffer (Transfer buffer)	T8052-050	500 ml
膜染色	Ponceau S Solution	P9100-010	100 ml

4. Western Blot

类别	产品名称	产品货号	规格
Blocking	Albumin, Ultra pure Bovine Serum Albumin	A0100-010	100 g
	20X TBS Buffer, pH 7.4	T8054-050	500 ml
	10X TBST Buffer, pH 7.4	T8056-100	1L
	Tween 20, Molecular Biology Grade	T9100-010	100 ml
	Incubation Case (5 slots)	DWG003	1 EA
二抗	Goat anti-Mouse IgG(H+L)-HRP, 1 mg/ml	SA001-500	500 μl
	Goat anti-Rabbit IgG(H+L)-HRP, 1 mg/ml	SA002-500	500 μl
	Rabbit anti-Goat IgG(H+L)-HRP, 1 mg/ml	SA007-500	500 μl

5. Detection

类别产品名称货号规格

ECL West-Q Chemiluminescent Substrate Kit, Plus W3651-012 120 ml

West-Q Pico ECL Solution W3652-020 200 ml

West-Q Pico Dura ECL Solution W3653-020 200 ml

West-Q Femto Clean ECL Solution W3680-010 100 ml

West-Q ECL Selection guide

产品名称	货号	产品特点	检测范围
West-Q Chemiluminescent Substrate Plus Kit	W3651	High sensitivity & stable signalsEconomical price	Picogram
West-Q Pico ECL Solution	W3652	High sensitivity & stable signalLow background	Low Picogram
West-Q Pico Dura ECL Solution	W3653	High Sensitivity & Stable SignalExcellent Signal Persistence	Low Picogram
West-Q Femto Clean ECL Solution	W3680	Very high sensitivity & stable signalsVery low background	Femtogram