CD14 ELISA

• 检测方法

  Colorimetric

• **度

  批次内

  样品	n	Mean	SD	CV%
  Sample A	8	210.5ng/ml	2.6	1.23%
  Sample B	8	23.4ng/ml	1.14	4.6%

  批次间

  样品	n	Mean	SD	CV%
  Sample A	8	210.5ng/ml	1.55	0.73%
  Sample B	8	24	2	8.3%

• 样品类型

  Cell culture supernatant, Milk, Serum, Plasma, Cell Lysate

• 检测类型

  Sandwich (quantitative)

• 灵敏度

  < 6 ng/ml

• 范围

  6.8 ng/ml - 220 ng/ml

• 检测时间

  3h 45m

• 实验步骤

  Multiple steps standard assay

• 种属反应性

  与反应: Human
  

• 产品概述

  Abcam’s CD14 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD14 in Human serum, plasma, buffered solutions or cell culture media.

  
  

  A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD14 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD14 present in the samples.

  
  

  This kit will recognize both endogenous and recombinant Human CD14.

  
  

  Get higher sensitivity in only 90 minutes with Human CD14 ELISA Kit (ab208983) from our SimpleStep ELISA® range.