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品牌：DiscoverX
品名：pCMV- ProLink™ 2 Vector 
货号：93-0171 
规格：1 Vector 

DiscoverX cell-based assays feature the powerful in vivo application of the established Enzyme Fragment Complementation technology. In this approach, two complementing fragments (ProLink, a small enzyme donor peptide fragment, and EA, a larger enzyme acceptor fragment) of the β-galactosidase (β-gal) enzyme are expressed as protein fusions in stably transfected, clonally derived cells. The pCMV-ProLink™ cloning vectors are intended for cloning a GPCR or other protein of interest with ProLink as a C-terminal tag, and subsequently transfecting into a parental cell line containing an EA-tagged protein. ProLink 1 peptide tag has low affinity for EA and is useful for strong protein:protein interactions, while ProLink 2 peptide tag has a higher affinity (~3-fold greater) for EA and is useful for weak protein:protein interactions. ARMS (Arrestin Recruitment Modifying Sequence) ProLink tags contain GRK (G-protein Receptor Kinase) consensus phosphorylation sites and can improve assays that have a low signal-to-background ratio.

Description

pCMV-ProLink™ 1 Vector

Contents

1 vial, 10µg in 20µL

Target

PK1

Compound

Compound

Compound Type

CompoundType

Storage

Ships frozen on dry ice. Store at –20°C until use.

Molecular WT (g/mole)

MolecularWTgmole

Source

Source