Catalogue number
IHA-022
Introduction
H1N1 is a subtype specie of Influenza A virus. H1N1 Influenza Virus has mutated into various strains such as the Spanish Flu strain, mild human flu strains, endemic pig strains, and various strains found in birds.
The Influenza A Virus is a globular particle about 100nm in diameter, sheathed in a lipid bilayer derived from the plasma membrane of its host. Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin ("H") and some 100 molecules of neuraminidase ("N"). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some "non-structural" protein molecules of uncertain function.
Description
Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza A virus, strain A/Solomon Islands/03/06. The Influenza Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.
Inactivation
Thimerosal and beta propiolactone treatment.
This product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are required at work.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The H1N1 A/Solomon Islands/03/06 solution contains 0.1M NaCl, 10mM Tris-HCl, 1mM EDTA pH-8 (STE), 0.1% sodium azide (NaN3) and 0.005% thimerosal.
Stability
A/Solomon Islands/03/06 although stable 4°C for 4 weeks, should be stored below -18°C.
Please prevent freeze-thaw cycles.
Immunological Activity
Tested with anti-influenza A monoclonal antibodies in ELISA.
Serological studies of influenza A virus, immunogen for antibody production.
Purity
Greater than 90.0% as determined by SDS-PAGE.
References
Title: Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine
Publication: The Journal of clinical investigation 120.5 (2010): 1663.
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860935/
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
