Catalogue number
PRO-795
Synonyms
LSM5 homolog, U6 small nuclear RNA associated
(S.cerevisiae), YER146W, U6 snRNA-associated Sm-like protein LSm5.
Introduction
LSM5
also known as LSM5 Homolog, U6 Small Nuclear RNA Associated belongs to the Sm
protein family. This family is identified in a variety of organisms
based on sequence homology. Sm-like proteins contain the Sm sequence motif,
which consists of 2 regions separated by a linker of variable length which
folds as a loop. In addition, the Sm-like proteins are thought to create a
stable heteromer present in tri-snRNP
particles, which are important for pre-mRNA splicing. Among the diseases
associated with LSM5 are spinal muscular atrophy, and muscular atrophy.
Description
LSM5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids (1-91 a.a) and having a molecular mass of 12.3kDa.
LSM5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
LSM5 protein solution (1mg/ml) containing 20mM Tris-HCl (pH
8.5), 10% glycerol, 0.15M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAANATT NPSQLLPLEL VDKCIGSRIH IVMKSDKEIV GTLLGFDDFV
NMVLEDVTEF EITPEGRRIT KLDQILLNGN NITMLVPGGE GPEV
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
