Catalogue number
PRO-1774
Synonyms
Sirtuin 5, NAD-Dependent Lysine Demalonylase And
Desuccinylase Sirtuin-5,Mitochondria, NAD-Dependent Protein Deacylase Sirtuin-5
Mitochondrial, Silent Mating Type Information Regulation 2 (S.Cerevisiae)
Homolog 5, NAD-Dependent Deacetylase Sirtuin-5, Regulatory Protein SIR2 Homolog
5, SIR2-Like Protein 5, SIR2L5, Sirtuin Type 5, Sir2-Like 5, EC 3.5.1.-.
Introduction
SIRT5 belongs to the Sirtuin family of proteins, homologs to the yeast Sir2 protein. Sirtuin family members hold a sirtuin core domain and grouped into four classes. The protein encoded by this gene is included in class III of the sirtuin family. Human sirtuins functions were not yet determined; still, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies propose that human sirtuin could operate as intracellular regulatory protein with mono-ADP-ribosyltransferase activity.
Description
SIRT5 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain topological domain containing 300 amino
acids (34-310 a.a) and having a molecular mass of 32.5kDa. SIRT5 is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SIRT5 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKW
QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG
TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV
VWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ
GPCGTTLPEA LACHENETVS
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
