Catalogue number
ENZ-362
Synonyms
ECK0697, JW0698, phrB, phr, Deoxyribodipyrimidine
photo-lyase, DNA photolyase, Photoreactivating enzyme, EC=4.1.99.3, b0708.
Introduction
Deoxyribodipyrimidine photo-lyase (phrB) is a member of the DNA
photolyase class-1 family. phrB takes part in repair of UV radiation-induced
DNA damage. phrB catalyzes the light-dependent monomerization (300-600
nm) of cyclobutyl pyrimidine dimers (in
cis-syn configuration), which are formed between closest bases on the same DNA
strand upon exposure to
ultraviolet radiation.
Description
phrB E.Coli Recombinant produced in E.coli is a single,
non-glycosylated polypeptide chain containing 495 amino acids (1-472) and
having a molecular mass of 56.1kDa.phrB is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The phrB solution (0.25mg/ml) contains 20mM Tris-HCl
buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMTTHLVW FRQDLRLHDN LALAAACRNS SARVLALYIA TPRQWATHNM
SPRQAELINA QLNGLQIALA EKGIPLLFRE
VDDFVASVEI VKQVCAENSV THLFYNYQYE VNERARDVEV ERALRNVVCE GFDDSVILPP GAVMTGNHEM
YKVFTPFKNA WLKRLREGMP ECVAAPKVRS SGSIEPSPSI TLNYPRQSFD TAHFPVEEKA
AIAQLRQFCQ NGAGEYEQQR DFPAVEGTSR LSASLATGGL
SPRQCLHRLL AEQPQALDGG AGSVWLNELI WREFYRHLIT YHPSLCKHRP FIAWTDRVQW
QSNPAHLQAW QEGKTGYPIV DAAMRQLNST GWMHNRLRMI TASFLVKDLL IDWREGERYF MSQLIDGDLA ANNGGWQWAA
STGTDAAPYF RIFNPTTQGE KFDHEGEFIR QWLPELRDVP
GKVVHEPWKW AQKAGVTLDY PQPIVEHKEA RVQTLAAYEA ARKGK.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
