Catalogue number
ENZ-672
Synonyms
Pyroglutamyl-peptidase 1, EC 3.4.19.3, 5-oxoprolyl-peptidase, Pyroglutamyl aminopeptidase I, PAP-I, Pyroglutamyl-peptidase I, PGP-I, Pyrrolidone-carboxylate peptidase, PGPEP1, PGPI, PGP, Pcp.
Introduction
Pyroglutamyl-Peptidase I (PGPEP1) is an omega peptidase which detaches pyroglutamyl residues from the amino termini of peptides and proteins. PGPEP1 is a cytosolic cysteine peptidase which is expressed in most cell types. PGPEP1 enzyme has need of s a thiol-reducing agent for activity. PGPEP1 is possibly involved in the inactivation of biologically active peptides which have an amino terminal pyroglutamyl group, for instance peptides as neurotensin, luteinizing hormone releasing hormone, and thyrotropinreleasing hormone.
Description
PGPEP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-209) and having a molecular mass of 25.5kDa.
PGPEP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The PGPEP1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMEQPRKA VVVTGFGPFG EHTVNASWIA VQELEKLGLG DSVDLHVYEI PVEYQTVQRL IPALWEKHSP QLVVHVGVSG MATTVTLEKC GHNKGYKGLD NCRFCPGSQC CVEDGPESID SIIDMDAVCK RVTTLGLDVS VTISQDAGRY LCDFTYYTSL YQSHGRSAFV HVPPLGKPYN ADQLGRALRA IIEEMLDLLE QSEGKINYCH KH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
