Catalogue number
ENZ-836
Synonyms
NT5C3L, 7-methylguanosine phosphate-specific
5'-nucleotidase, Cytosolic 5'-nucleotidase 3B, Cytosolic 5'-nucleotidase
III-like protein, cN-III-like protein, N(7)-methylguanylate 5'-phosphatase.
Introduction
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) includes transferase activity and nucleotide binding. Among NT5C3B’s related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. NT5C3B hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP guards cells against undesired retrievals of m(7)GMP and its incorporation into nucleic acids. In addition, NT5C3B has weak activity for CMP.
Description
NT5C3B Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 323 amino acids (1-300 a.a) and having
a molecular mass of 36.8kDa.NT5C3B is fused
to a 23 amino acid His-tag at N-terminus & purified by proprietary
chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
NT5C3B protein solution (1mg/ml) containing Phosphate
Buffered Saline (pH7.4), 20% glycerol and
1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS
RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK
IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN
EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV
EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
