Catalogue number
ENZ-264
Synonyms
Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.
Introduction
Urokinase (UK) is a serine protease, which is one of biological plasminogen activators.
It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing.
It can be obtained from human urine or kidney cell culture.
Description
Urokinase is a two-chain glycoprotein containing 411 amino acids with 12 disulfide bonds. Its molecular weight is 54,000 Dalton.
Source
Human urine.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The Urokinase was lyophilized from a concentrated (1mg/ml) solution containing phosphate buffer.
Solubility
It is recommended to reconstitute the lyophilized Urokinase in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Urokinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Urokinase should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity
Greater than 92.0% as determined by SDS-PAGE.
Specific Activity
Contaminants
Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
References
1.
Title:Transient ?-catenin stabilization modifies lineage output from human thymic CD34
+CD1a
– progenitors.
Publication:Published online before print December 1, 2009, doi: 10.1189/jlb.0509344
March 2010 Journal of Leukocyte Biology vol. 87 no. 3 405-414 .
Link:http://www.jleukbio.org/content/87/3/405.full
2. Title: The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysin
Publications: Biochem. J 410 (2008): 157-165.
Link: http://www.biochemj.org/bj/410/bj4100157.htm
3. Title:Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa.
Publication:Biochem. J 428 (2010): 473-482.
Link:http://www.biochemj.org/bj/428/0473/4280473.pdf
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
