Catalogue number
PKA-319
Synonyms
MOB Kinase Activator 3B, Chromosome 9 Open Reading Frame 35, Monopolar Spindle 1 Binding, MOB1, Domain Containing, MOB1 Mps One Binder Kinase Activator-Like 2B, Mps One Binder Kinase Activator-Like 2B, Monopolar Spindle 1 Binding, Mob1 Homolog 2b, MOBKL2B, Domain Containing, C9orf35, MOB1D, MOB1.
Introduction
MOB3B is similar to yeast Mob1 protein which binds Mps1p, a protein kinase vital for spindle pole body duplication and mitotic checkpoint regulation. MOB3B is situated on the opposite strand as the INF kappa precursor (IFNK) gene.
Description
MOB3B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (1-216) and having a molecular mass of 27.9 kDa. MOB3B is fused to a 23 amino acid His-tag at N-terminus.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The MOB3B solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSIALKQ VFNKDKTFRP KRKFEPGTQR FELHKRAQAS LNSGVDLKAA VQLPSGEDQN DWVAVHVVDF FNRINLIYGT ICEFCTERTC PVMSGGPKYE YRWQDDLKYK KPTALPAPQY MNLLMDWIEV QINNEEIFPT CVGVPFPKNF LQICKKILCR LFRVFVHVYI HHFDRVIVMG AEAHVNTCYK HFYYFVTEMN LIDRKELEPL KEMTSRMCH
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
