Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.
Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosesynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.
Thyroid Peroxidase Human Recombinant produced in SF9 is a Biotinylated, glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 93 kDa (excluding glycosylation).
The TPO is expressed with a -6xHis tag at C-terminus and purified by proprietary chromatographic techniques.
Sf9 insect cells.
Sterile Filtered clear solution.
TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80% as determined by SDS-PAGE.
1. Binds IgG-type human auto-antibodies.
2. Auto-antibodies to TPO recognize conformation-dependent epitopes.
3. Functional Streptavidin based ELISA test (analysis of positive/negative samples.)
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
