Catalogue number
ENZ-902
Synonyms
Carbonic Anhydrase
XIII, Carbonate Dehydratase XIII, EC 4.2.1.1, CA-XIII, Carbonic, Anhydrase 13, CAXIII.
Introduction
Carbonic Anhydrase XIII, also known as CA13 is a member of the
alpha-carbonic anhydrase family, which catalyzes the rapid interconversion of
carbon dioxide and water to bicarbonate and
protons, a reversible reaction which occurs relatively slowly in the absence of
catalyst. Furthermore, the active site of nearly all
carbonic anhydrases contains a zinc ion; they have been classified as
metalloenzymes. At least five distinct CA families
(?, ?, ?, ? and ?) have been found. These families have no significant a.a
sequence resemblance and in just about all cases are considered
to be an example of convergent evolution. The ?-CAs have been demonstrated in
humans.
Description
CA13 Human
Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain
containing 285 amino acids (1-262a.a) and having a molecular mass of 31.8kDa.
CA13 is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CA13 protein
solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4),10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as
determined by SDS-PAGE.
Biological Activity
Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSP
MGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF
NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH
EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES
VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
