Catalogue number
ANT-191
Synonyms
B1, S7, Bp35, MS4A2, LEU-16, MGC3969.
Type
Anti Human Recombinant.
Introduction
CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 plays a role in the development and differentiation of B-cells into plasma cells. CD20 is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.
Description
Recombinant Anti Human CD20 monoclonal Antibody produced in CHO is a glycosylated polypeptide chain having a molecular mass of 80 kDa.
CD-20 is purified by proprietary chromatographic techniques.
Source
CHO.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized rAHuCD-20 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized CD-20 Recombinant Antibody although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution CD20 Antibody should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by SDS-PAGE gel.
Biological Activity
Fully biologically active when compared to standard determined by the apoptosis assay of Raji cell in vitro.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
