Catalogue number
PRO-994
Synonyms
Small nuclear ribonucleoprotein Sm D1, Sm-D1, Sm-D autoantigen, snRNP core protein D1, SNRPD1, SMD1, SNRPD, HsT2456.
Introduction
SNRPD1 is a small nuclear ribonucleoprotein which is a member of the SNRNP core protein family. SNRPD1 may act as a charged protein scaffold to stimulate SNRNP assembly or strengthen SNRNP-SNRNP interactions via nonspecific electrostatic contacts with RNA. In the autoimmune disease systemic lupus erythematosus, antinuclear antibodies are developed with Sm specificity.
Description
SNRPD1 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,306 Dalton.
SNRPD1 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Sf9 insect cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SNRPD1 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Coating concentration
0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Applications
Western blot with anti-Sm positive patient sera.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
