Catalogue number
PRO-1468
Synonyms
15 KDa Selenoprotein, SEP15.
Introduction
15 KDa Selenoprotein (SEP15) is a
protein-coding gene which contains a selenocysteine (Sec) residue at its active
site. The selenocysteine
is encoded by the UGA codon that usually signals translation termination. The
3' UTR of selenoprotein
genes have a conventional stem-loop structure, the sec insertion sequence
(SECIS), which is necessary
for the recognition of UGA as a Sec codon rather than as a stop signal. Studies
in mouse propose that this
selenoprotein may have redox function and may be implicated in the quality
control of protein folding. This gene is localized on chromosome 1p31, a genetic locus usually
mutated or deleted in human cancers.Diseases associated with SEP15 include lung cancer susceptibility, and
chronic lymphocytic leukemia.
Description
SEP15 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (29-165 a.a) and having a molecular mass of 17.7kDa.
SEP15 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SEP15 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSVSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ
EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS
ILKWNTDSVE EFLSEKLERI
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
