Catalogue number
PRO-2369
Synonyms
R-spondin-1, Cristin-3, mCristin-3, Roof plate-specific
spondin-1, Rspo1, RSPO, R-spondin 1,
R-spondin, Rspondin, CRISTIN3.
Introduction
R-Spondin-1
(Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator of
the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors.
Rspo1 induces the onset of crypt cell proliferation and increases intestinal
epithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway.
Description
RSPO1 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 254 amino acids (21-265a.a.) and
having a molecular mass of 28.2kDa (Molecular size on SDS-PAGE will appear at
approximately 28-40kDa).
RSPO1 is expressed with an 9 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RSPO1 protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPSRGIKGK
RQRRISAEGS QACAKGCELC SEVNGCLKCS PKLFILLERN DIRQVGVCLP SCPPGYFDAR NPDMNKCIKC
KIEHCEACFS HNFCTKCQEG LYLHKGRCYP ACPEGSTAAN STMECGSPAQ CEMSEWSPWG PCSKKRKLCG
FRKGSEERTR RVLHAPGGDH TTCSDTKETR KCTVRRTPCP EGQKRRKGGQ GRRENANRHP ARKNSKEPGS
NSRRHKGQQQ PQPGTTGPLT SVGPTWAQHH HHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
