Catalogue number
PRO-1733
Synonyms
Phospholipid scramblase 3, PL scramblase 3, Ca(2+)-dependent
phospholipid scramblase 3.
Introduction
Phospholipid
Scramblase 3, (PLSCR3) may mediate accelerated ATP-independent bidirectional
transbilayer migration of phospholipids upon binding calcium ions which results
in a loss of phospholipid asymmetry in the plasma membrane. PLSCR3
may play a key role in the initiation of fibrin clot formation, in the
activation of mast cells and in the recognition of apoptotic and injured cells
by the reticuloendothelial system. In addition, PLSCR3 seems to play a role in
apoptosis, through
translocations of cardiolipin from the inner to the outer mitochondrial membrane
which promotes BID recruitment and enhances tBid-induced mitochondrial damages.
Among the diseases associated with PLSCR3 are brain ischemia, and ischemia.
Description
PLSCR3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids (1-265 a.a) and having a molecular mass of 30.9kDa.
PLSCR3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
PLSCR3 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 10% glycerol and 0.4M urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGYLPP KGYAPSPPPP YPVTPGYPEP ALHPGPGQAP VPAQVPAPAP
GFALFPSPGP VALGSAAPFL PLPGVPSGLE FLVQIDQILI HQKAERVETF LGWETCNRYE LRSGAGQPLG
QAAEESNCCA RLCCGARRPL RVRLADPGDR EVLRLLRPLH CGCSCCPCGL QEMEVQAPPG TTIGHVLQTW HPFLPKFSIQ DADRQTVLRV
VGPCWTCGCG TDTNFEVKTR DESRSVGRIS KQWGGLVREA LTDADDFGLQ FPLDLDVR
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
