Catalogue number
PRO-1764
Synonyms
Vav 1 Guanine Nucleotide Exchange Factor, VAV, Vav 1
Oncogene, Proto-Oncogene Vav.
Introduction
Vav
1 Guanine Nucleotide Exchange Factor (VAV1), belongs to the VAV gene family.
The VAV1 proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases which
activate pathways leading to actin cytoskeletal rearrangements and transcriptional
alterations. VAV1 is essential in hematopoiesis, taking part in T-cell and
B-cell development and activation. VAV1 has been identified as the specific
binding partner of Nef proteins from HIV-1. In addition, VAV1 contains an SH2
domain, which could show its function as a substrate for tyrosine kinases. Expression
of VAV1 is limited solely to cells of hematopoietic origin, as well as those of
the erythroid, lymphoid and myeloid lineages. Among the diseases associated
with VAV1 are cherubism, and hiv-1.
Description
VAV1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (189-565a.a) and having a molecular mass of 46.8kDa.
VAV1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
VAV1 protein solution (0.25mg/ml) containing In 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30%
glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMTEYDKR CCCLREIQQT EEKYTDTLGS IQQHFLKPLQ RFLKPQDIEI
IFINIEDLLR VHTHFLKEMK EALGTPGAAN LYQVFIKYKE RFLVYGRYCS QVESASKHLD RVAAAREDVQ
MKLEECSQRA NNGRFTLRDL LMVPMQRVLK YHLLLQELVK HTQEAMEKEN LRLALDAMRD LAQCVNEVKR DNETLRQITN FQLSIENLDQ
SLAHYGRPKI DGELKITSVE RRSKMDRYAF LLDKALLICK RRGDSYDLKD FVNLHSFQVR DDSSGDRDNK
KWSHMFLLIE DQGAQGYELF FKTRELKKKW MEQFEMAISN IYPENATANG HDFQMFSFEE TTSCKACQML LRGTFYQGYR CHRCRASAHK ECLGRVPPCG
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
