Catalogue number
PRO-1887
Synonyms
CD33Molecule, CD33Antigen (Gp67), Sialic Acid
Binding Ig-Like Lectin 3, Sialic Acid-Binding Ig-Like Lectin 3, SIGLEC3, gp67, SIGLEC-3,
Myeloid Cell Surface AntigenCD33, p67, Siglec-3, CD33Antigen.
Introduction
CD33
is putative adhesion molecule of myelomonocytic-derived cells which mediates
sialic-acid dependent binding to cells. CD33 prefers to bind to
alpha-2,6-linked sialic acid. The sialic acid recognition site is masked by cis
interactions with sialic acids on the same cell surface. In the immune
response, CD33 perform as an inhibitory receptor upon ligand induced tyrosine
phosphorylation by recruiting cytoplasmic phosphatases through their SH2
domains which block signal transduction through dephosphorylation of signaling
molecules. In addition, CD33 induces apoptosis in acute
myeloid leukemia.
Description
CD33 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 265 amino acids (18-259 a.a) and
having a molecular mass of 29.1kDa.
CD33 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CD33 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSDPNFWLQ VQESVTVQEG LCVLVPCTFF HPIPYYDKNS PVHGYWFREG AIISGDSPVA TNKLDQEVQE ETQGRFRLLG DPSRNNCSLS IVDARRRDNG SYFFRMERGS TKYSYKSPQL SVHVTDLTHR PKILIPGTLE PGHSKNLTCS VSWACEQGTP PIFSWLSAAP TSLGPRTTHS SVLIITPRPQ DHGTNLTCQV KFAGAGVTTE RTIQLNVTYV PQNPTTGIFP GDGSGKQETR AGVVH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
