Catalogue number
CYT-368
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Introduction
Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
Description
TGF-β 3 Human Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing two 113 amino acid chains and having a total molecular mass of 25.8kDa.
The TGF-β 3 is purified by standard chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The protein solution contains 20% Ethanol and 10mM Acetic acid (AcOH).
Stability
TGF-beta 3 although stable at room temperature for 1 week, should be stored at 4°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS.
Biological Activity
The activity is determined by the ability to induce chondrogenic differentiation.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.
References
1.
Title: Altering the Architecture of Tissue Engineered Hypertrophic Cartilaginous Grafts Facilitates Vascularisation and Accelerates Mineralisation
Publication: DOI: 10.1371/journal.pone.0090716, March 04, 2014
Link: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0090716
2. Title: Oxygen tension differentially regulates
the functional properties of cartilaginous tissues engineered from
infrapatellar fat pad derived MSCs and articular chondrocytes
Publication: Osteoarthritis and Cartilage 18.10 (2010):
1345-1354.
Link: http://www.sciencedirect.com/science/article/pii/S1063458410002293
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
