Catalogue number
PRO-2183
Synonyms
Jumonji Domain Containing 6, Phosphatidylserine Receptor,
Jumonji Domain-Containing Protein 6, Histone Arginine Demethylase JMJD6, Peptide-Lysine
5-Dioxygenase JMJD6, JmjC Domain-Containing Protein 6, Lysyl-Hydroxylase JMJD6,
PTDSR, PSR, Bifunctional Arginine Demethylase And Lysyl-Hydroxylase JMJD6,
Protein PTDSR, EC 1.14.11.-, EC 1.14.11, KIAA0585, PTDSR1, Bifunctional
arginine demethylase and lysyl-hydroxylase JMJD6 (EC:1.14.11.-).
Introduction
Jumonji Domain Containing 6, also known as JMJD6 performs as a
lysyl-hydroxylase which catalyzes 5-hydroxylation on specific lysine residues
of target proteins for instance U2AF2/U2AF65 and LUC7L2, as a regulator of RNA splicing by mediating 5-hydroxylation of
U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. Additionally
to peptidyl-lysine 5-dioxygenase activity, JMJD6 performs as an RNA
hydroxylase, as proposed by its ability to bind single strand RNA. Furthermore,
it acts as an arginine demethylase which demethylates histone H3 at 'Arg-2'
(H3R2me) as well as histone H4 at 'Arg-3' (H4R3me), thus taking a part in histone code.
Description
JMJD6 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 437 amino acids (1-414 a.a) and
having a molecular mass of 50.0kDa.
JMJD6 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
JMJD6 protein solution (0.5mg/ml) containing Phosphate
buffered saline (pH7.4), 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSMNHKSKK
RIREAKRSAR PELKDSLDWT RHNYYESFSL SPAAVADNVE RADALQLSVE EFVERYERPY KPVVLLNAQE GWSAQEKWTL
ERLKRKYRNQ KFKCGEDNDG YSVKMKMKYY IEYMESTRDD SPLYIFDSSY GEHPKRRKLL EDYKVPKFFT
DDLFQYAGEK RRPPYRWFVM GPPRSGTGIH IDPLGTSAWN ALVQGHKRWC LFPTSTPREL IKVTRDEGGN
QQDEAITWFN VIYPRTQLPT WPPEFKPLEI LQKPGETVFV PGGWWHVVLN LDTTIAITQN FASSTNFPVV
WHKTVRGRPK LSRKWYRILK QEHPELAVLA DSVDLQESTG IASDSSSDSS SSSSSSSSDS DSECESGSEG
DGTVHRRKKR RTCSMVGNGD TTSQDDCVSK ERSSSRIRDT CGGRAHP.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
