Catalogue number
PRO-2099
Synonyms
Mitochondrial Ribosomal Protein L48, MRP-L48, L48MT, 39S
Ribosomal Protein L48, Mitochondrial, CGI-118, HSPC290, 39S ribosomal protein
L48, mitochondrial.
Introduction
Mitochondrial Ribosomal Protein L48, also known as MRPL48, is a
mammalian mitochondrial ribosomal protein which assists in protein synthesis
within the mitochondrion. Mitochondrial ribosomes, mitoribosomes, consist of a
small 28S subunit and a large 39S subunit. They include an estimated 75%
protein to rRNA composition while comparing to prokaryotic ribosomes, where
this ratio is reversed.An additional dissimilarity between mammalian mitoribosomes & prokaryotic
ribosomes is that the latter contain a 5S rRNA. Between different species, the
proteins containing the mitoribosome differ very much in sequence, as well as in
biochemical properties from time to time, which prevents easy recognition through
sequence homology. MRPL48 encodes a 39S subunit protein. A pseudogene
corresponding to MRPL48 is found on chromosome 6p.
Description
MRPL48 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 207 amino acids (29-212 a.a) and
having a molecular mass of 23.1kDa.
MRPL48 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
MRPL48 protein solution (1 mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSSGEKPIY SVGGILLSIS RPYKTKPTHG IGKYKHLIKA EEPKKKKGKV
EVRAINLGTD YEYGVLNIHL TAYDMTLAES YAQYVHNLCN SLSIKVEESY AMPTKTIEVL QLQDQGSKML
LDSVLTTHER VVQISGLSAT FAEIFLEIIQ SSLPEGVRLS
VKEHTEEDFK GRFKARPELE ELLAKLK.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
