Catalogue number
ENZ-861
Synonyms
Protein phosphatase 1D magnesium-dependent, delta isoform, PPM1D, Protein Phosphatase 1D, PP2C-DELTA, WIP1.
Introduction
Protein
Phosphatase 1D (PPM1D) belongs to the PP2C family of Ser/Thr protein
phosphatases whose members are negative regulators of cell stress response
pathways. PPM1D is induced by tumor suppressor protein TP53/p53 in response to multiple
environmental stresses. PPM1D is a negative regulator of the activity of p38
MAP kinase through which it reduces the phosphorylation of p53 and suppresses
p53-mediated transcription and apoptosis. PPM1D is located in a chromosomal area
which enlarges in breast cancer.
Description
PPM1D Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (98-375a.a.) and having a molecular mass of 33.2kDa.
PPM1D is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
PPM1D protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MVAFFAVCDG HGGREAAQFA REHFWGFIKK QKGFTSSEPA KVCAAIRKGF LACHLAMWKK LAEWPKTMTG LPSTSGTTAS VVIIRGMKMY VAHVGDSGVV LGIQDDPKDD FVRAVEVTQD HKPELPKERE RIEGLGGSVM NKSGVNRVVW KRPRLTHNGP VRRSTVIDQI PFLAVARALG DLWSYDFFSG EFVVSPEPDT SVHTLDPQKH KYIILGSDGL WNMIPPQDAI SMCQDQEEKK YLMGEHGQSC AKMLVNRALG RWRQRMLRAD NTSAIVICI.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
