Catalogue number
PRO-954
Synonyms
S3, Ribosomal protein S3, 40S ribosomal protein S3, IMR-90 ribosomal protein S3, MGC87870, FLJ26283, FLJ27450.
Introduction
40S ribosomal protein S3 is a member of the S3P family of ribosomal proteins. RPS3 facilitates the formation of the mRNA 3' of the codon in the decoding site. Moreover, RPS3 takes part in DNA damage recognition due to its affinity for abasic sites and 7, 8-dihydro-8-oxoguanine residues and its collaboration with human base excision repair proteins OGG1 and Ref-1. Expression of RPS3 is higher in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa.
Description
RPS3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (1-243) and having a molecular mass of 28.8 kDa.
RPS3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The RPS3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
