Catalogue number
PRO-303
Synonyms
Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.
Introduction
Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis.
Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10 % of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.
Description
PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35 kDa.
PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Source
Sf9 insect cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.
Applications
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration
0.5-1.1 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Purity
Greater than 95% as determined by SDS-PAGE.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
