Catalogue number
HSP-022
Synonyms
HSPB8, H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22, Heat shock protein beta-8, Alpha-crystallin C chain, Small stress protein-like protein HSP22, E2-induced gene 1 protein, Protein kinase H11, CRYAC.
Introduction
HSPB8 displays temperature-dependent chaperone activity. HSPB8 acts as a mn(2+)-dependent serine-threonine- specific protein kinase. we are not convinced that this is its true role. Defects in HSPB8 are a cause of distal hereditary motor neuropathy type ii (DHMN2) also known as distal spinal muscular atrophy (DSMA) and spinal muscular atrophy of the charcot-marie-tooth type. it is an autosomal dominant disorder of lower motor neurons characterized by distal muscle weakness.
Description
Recombinant Human Heat Shock Protein 22 kDa Protein-8 is a full-length human HSP22 with an MW of 21604 Dalton produced in E.coli.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The HSPB8 protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Tris-acetate, pH-7.6, 10mM NaCl, 0.1mM EDTA, 0.1mM PMSF, 15mM b-ME.
Solubility
It is recommended to reconstitute the lyophilized HSPB8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized HSPB8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSPB8 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Immunological Activity
Immunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSPB8.
References
Title: Roles of ERK and NF-?B in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells.
Publication: Korean J Physiol Pharmacol. 2008 Aug;12(4):171-176.
Link: http://synapse.koreamed.org/DOIx.php?id=10.4196/kjpp.2008.12.4.171&vmode=FULL
Applications: HSP22 induces IL-8 in VSMCs via ERK1/2.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
