Catalogue number
PRO-1740
Synonyms
Eukaryotic Translation Initiation Factor 3, Subunit I, EIF3S2,
Eukaryotic Translation Initiation Factor 3, Subunit 2 Beta, 36kDa, Eukaryotic
Translation Initiation Factor 3 Subunit 2, TRIP-1, eIF-3-beta, EIF3 P36, TGF-Beta
Receptor-Interacting Protein 1, PRO2242, eIF3-beta, eIF3-p36, Eukaryotic
Translation Initiation Factor 3 Subunit I, Eukaryotic Translation Initiation
Factor 3, Subunit 2 (Beta, 36kD), Predicted Protein Of HQ2242, TGFbeta
Receptor-Interacting Protein 1, eIF3i.
Introduction
Eukaryotic
translation initiation factor 3, subunit I (EIF3I) is part of the eukaryotic
translation initiation factor 3 (eIF-3) complex, which is essential for
numerous steps in the initiation of protein synthesis. The eIF-3 complex links
with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:
GTP: methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S
PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning
of the mRNA for AUG recognition. The eIF-3 complex is also essential for
disassembly and recycling of post-termination ribosomal complexes and
subsequently prevents premature joining of the 40S and 60S ribosomal subunits
prior to initiation. Among the diseases associated with EIF3I are clonorchiasis,
and tonsillitis.
Description
EIF3I Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 348 amino acids (1-325aa) and having a molecular mass of 38.9kDa.
EIF3I is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
APOBEC4 protein solution (1mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMKPILLQ GHERSITQIK YNREGDLLFT VAKDPIVNVW YSVNGERLGT
YMGHTGAVWC VDADWDTKHV LTGSADNSCR LWDCETGKQL ALLKTNSAVR TCGFDFGGNI IMFSTDKQMG
YQCFVSFFDL RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS GEVLVNVKEH SRQINDIQLS RDMTMFVTAS
KDNTAKLFDS TTLEHQKTFR TERPVNSAAL SPNYDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE
FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFEFEFEA
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
