Catalogue number
CMV-215
Introduction
CMV belongs to the Betaherpesvirinae subfamily of Herpesviridae which includes herpes simplex virustypes 1 and 2, varicella-zoster virus, and Epstein-Barrvirus. The herpesviruses share a characteristic ability to remain latentover long periods. CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early, delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts.
Description
The E.Coli derived 52.2 kDa recombinant protein contains the CMV Pp65 (UL83) immunodominant regions, 297-510 amino acids. Recombinant CMV-Pp65 is fused to a 26 kDa GST tag.
Source
Escherichia Coli.
Purification Method
CMV Pp65 was purified by proprietary chromatographic technique.
Purity
CMV Pp65 protein is >95% pure as determined by SDS-PAGE.
Formulation
50mM Tris-HCl, 10mM glutathione, 60mM NaCl, 0.125% sarcosil and 50% glycerol.
Stability
CMV Pp65 protein although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Specificity
Immunoreactive with sera of CMV-infected individuals.
Applications
CMV Pp65 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
References
Title: Cytomegalovirus-Infected Human
Endothelial Cells Can Stimulate Allogeneic CD4+ Memory T Cells by Releasing
Antigenic Exosomes
Publication: Endocrinology 150.7 (2009): 3252-3258.
Link: http://www.jimmunol.org/content/182/3/1548.full
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
