Catalogue number
ENZ-1016
Introduction
Chymotrypsinogen-B1 (CTRB1) belongs to the serine protease family of
enzymes and forms a main precursor of the pancreatic proteolytic enzymes. CTRB1
is located next to a related chymotrypsinogen gene. CTRB1 is a protein coding
gene which encodes different isoforms which may undergo similar processing to
generate the mature protein.
Description
Recombinant Human CTRB1 expressed in E.coli containing 245
amino acids having a Mw of 27kDa is purified
by standard chromatography techniques.
Source
E.coli
Physical Appearance
Sterile Filtered lyophilized powder.
Formulation
The Human CTRB1 was lyophilized without any additives.
Solubility
It is recommended to reconstitute the lyophilized Human CTRB1 in 1ml 50mM HAc which can then be further diluted to other aqueous solutions.
Stability
Recombinant Human CTRB1 although stable at room temp for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95% as
determined by HPLC.
Biological Activity
1100 units/mg protein.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 μmole of N-alpha-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 7.0 at 25°C.
Amino acid sequence
CG VPAIHPVLSG LSRIVNGEDA VPGSWPWQVS LQDKTGFHFC GGSLISEDWV
VTAAHCGVRT SDVVVAGEFD QGSDEENIQV LKIAKVFKNP KFSILTVNND ITLLKLATPA RFSQTVSAVC
LPSADDDFPAGTLCATTGWG KTKYNANKTP DKLQQAALPL LSNAECKKSW GRRITDVMIC AGASGVSSCM
GDSGGPLVCQ KDGAWTLVGI VSWGSDTCST SSPGVYARVTKLIPWVQKIL AAN.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
