Catalogue number
ENZ-927
Synonyms
Carnosine
Dipeptidase 1 (Metallopeptidase M20 Family), Glutamate Carboxypeptidase-Like
Protein 2, CNDP Dipeptidase 1, Serum Carnosinase, Carnosinase 1, CPGL2, CN1,
Carnosine Dipeptidase 1, EC 3.4.13.20, HsT2308.
Introduction
CNDP Dipeptidase 1, also known as CNDP1 is a member of the peptidase
M20A family. CNDP1 Mannheim which is the shortest allelic form has been more
common in the absence of nephropathy in addition to being associated with lower serum carnosinase levels. Furthermore, Carnosine inhibited the increased
production of fibronectin as well as collagen type VI in podocytes and the
increased production of TGF-beta in mesangial cells. Diabetic patients with the
CNDP1 Mannheim variant are less at risk for nephropathy. In addition, on renal
cells carnosine protects against the adverse effects of high glucose levels.
Description
CNDP1 produced in Sf9
Insect cells is a single, glycosylated polypeptide chain containing 489 amino
acids (27-507a.a.) and having a molecular mass of 54.9kDa. (Molecular size on
SDS-PAGE will appear at approximately 50-70kDa).
CNDP1 is expressed with
an 8 amino acid His tag at C-Terminus and purified by proprietary
chromatographic techniques.
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Source
Sf9,
Insect cells.
Physical Appearance
Sterile
filtered colorless solution.
Formulation
CNDP1
protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and
10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
SPSPPPALLE KVFQYIDLHQ DEFVQTLKEW VAIESDSVQP VPRFRQELFR MMAVAADTLQ RLGARVASVD MGPQQLPDGQ SLPIPPVILA ELGSDPTKGT VCFYGHLDVQ PADRGDGWLT DPYVLTEVDG KLYGRGATDN KGPVLAWINA VSAFRALEQD LPVNIKFIIE GMEEAGSVAL EELVEKEKDR FFSGVDYIVI SDNLWISQRK PAITYGTRGN SYFMVEVKCR DQDFHSGTFG GILHEPMADL VALLGSLVDS SGHILVPGIY DEVVPLTEEE INTYKAIHLD LEEYRNSSRV
EKFLFDTKEE ILMHLWRYPS LSIHGIEGAF DEPGTKTVIP GRVIGKFSIR LVPHMNVSAV EKQVTRHLED VFSKRNSSNK MVVSMTLGLH PWIANIDDTQ YLAAKRAIRT VFGTEPDMIR DGSTIPIAKM FQEIVHKSVV LIPLGAVDDG EHSQNEKINR WNYIEGTKLF AAFFLEMAQL HLEHHHHHH.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
