Catalogue number
CKI-269
Synonyms
Creatine Kinase MB Isoenzyme Type-I, CKMBITI, CKMBI, CKMB.
Introduction
The three isoenzymes (MM, MB, and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine Kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle's disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other that create elevated or reduced levels of Creatine Kinases.
Description
CKMBITI Human Recombinant produced in Pichia Pastoris is a glycosylated polypeptide chain and a full length Creatine Kinase MB isoenzyme, which is missing the C-terminal Lysine on the M subunit. CKMBITI is a dimeric protein comprised of M and B subunits, having a total Mw of ~44kDa.
The CKMBITI is purified by proprietary chromatographic techniques.
Source
Pichia Pastoris.
Physical Appearance
Sterile Filtered colorless liquid formulation.
Formulation
The 7.9mg/ml protein solution contains 0.02M Potassium Phosphate, 1mM DTT and 50% glycerol, pH 5.0-6.0.
Stability
CKMBITI although stable at 15°C for 7 days, should be stored below -18°C.
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
486 IU/mg at 37°C.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
