Catalogue number
ENZ-776
Synonyms
Cathepsin
E, EC 3.4.23.34, CATE, Erythrocyte Membrane Aspartic Proteinase, Slow-Moving
Proteinase, EC 3.4.23.
Introduction
Cathepsin-E also known as CTSE is a gastric aspartyl protease which
functions as a disulfide-linked homodimer. CTSE belongs to the peptidase C1 family;
furthermore it has specificity similar to pepsin A and cathepsin D. CTSE is an
intracellular proteinase which does not seem to be involved in the digestion of
dietary protein and is found in the
uppermost concentration in the surface
of epithelial mucus-producing cells of the stomach. CTSE is the first aspartic proteinase expressed in the fetal stomach and is discovered in more
than half of gastric cancers. For that reason CTSE is an oncofetal
antigen. In addition, transcript variants utilizing alternative polyadenylation
signals and two transcript variants encoding different
isoforms exist for this gene.
Description
CTSE Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 330 amino acids (57-363 a.a) and
having a molecular mass of 35.4kDa.
CTSE is fused to a 23 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CTSE protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.4M urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSTESCSMD QSAKEPLINY LDMEYFGTIS IGSPPQNFTV IFDTGSSNLW
VPSVYCTSPA CKTHSRFQPS QSSTYSQPGQ SFSIQYGTGS LSGIIGADQV SVEGLTVVGQ QFGESVTEPG
QTFVDAEFDG ILGLGYPSLA VGGVTPVFDN MMAQNLVDLP MFSVYMSSNP EGGAGSELIF GGYDHSHFSG SLNWVPVTKQ AYWQIALDNM
LWSVPTLTSC RMSPSPLTES PIPSAQLPTP YWTSWMECSS AAVAFKDLTS TLQLGPSGSW GMSSFDSFTQ
SLTVGITVWD WPQQSPKEGP CVCACLSDRP
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
