Streptolysin O, Thiol-activated cytolysin, slo.
Streptolysin-O is a sulfhydryl-activated toxin which causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the Streptolysin-O protein undergoes a major conformation change, leading to its insertion in the host membrane and creation of an oligomeric pore complex. Cholesterol may be needed for binding to host membranes, membrane insertion and pore formation. Streptolysin-O can be reversibly inactivated by oxidation.
Recombinant Streptolysin-O produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 61kDa.
Streptolysin-O is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Escherichia Coli.
Sterile Filtered clear solution.
Streptolysin-O is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
1. Binds IgG- and IgM-type human antibodies.
2. Immunodot test with positive/negative samples.
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