Catalogue number
CYT-227
Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor.
Introduction
A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
Description
Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16 kDa.
The Leptin is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Solubility
It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His.
Biological Activity
Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
References
1.Title:Expression of Hypothalamic KiSS-1 System and Rescue of Defective Responses by Kisspeptin in Streptozotocin-Induced Diabetic Male Rats.
Publication: doi: 10.2337/db05-1584 Diabetes September 2006 vol. 55 no. 9 2602-2610
Link:http://diabetes.diabetesjournals.org/content/55/9/2602.full?patientinform-links=yes&legid=diabetes;55/9/2602
2.Title:Desensitization of responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states.
Publication:Am J Physiol Endocrinol Metab 294: E1088–E1096, 2008.294: E1088–E1096, 2008. First published April 15, 2008; doi:10.1152/ajpendo.90240.2008.
Link:http://ajpendo.physiology.org/content/294/6/E1088.full.pdf
3.Title:p38 MAPK and LXR? mediate leptin effect on SREBP-1c expression in hepatic stellate cells (short title: Leptin effect on SREBP-1c expression) .
Publication:
Link:http://www.molmed.org/content/papers%20in%20press/11_243_Yan.pdf
4.Title:Neuroprotection by leptin in a rat model of permanent cerebral ischemia: effects on STAT3 phosphorylation in discrete cells of the brain.
Publication:Citation: Cell Death and Disease (2011) 2, e238; doi:10.1038/cddis.2011.125
Published online 8 December 2011.
Link:http://www.nature.com/cddis/journal/v2/n12/full/cddis2011125a.html
5.Title: The mammalian target of rapamycin as novel central regulator of puberty onset via modulation of hypothalamic Kiss1 system.
Publication: Endocrinology 150.11 (2009): 5016-5026.
Link http://press.endocrine.org/doi/full/10.1210/en.2009-0096
