Catalogue number
CYT-949
Synonyms
Leukemia Inhibitory Factor Receptor Alpha, CD118 Antigen,
LIF Receptor, LIF-R, Leukemia Inhibitory Factor Receptor, CD118, SJS2, STWS, SWS,
Leukemia inhibitory factor receptor, LIF receptor, LIF-R.
Introduction
Leukemia inhibitory factor receptor (LIFR) is the receptor for leukemia inhibitory factor, a pleiotropic cytokine affecting the differentiation, survival, and proliferation of various cells in the ***** and the embryo. LIFR plays an imperative role in a number of aspects of early pregnancy such as blastocyst implantation in the uterus.
Description
LIFR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 798 amino acids (45-833a.a.) and having a molecular mass of 90.5kDa (Molecular size on SDS-PAGE will appear at approximately 100-150kDa).
LIFR is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile
filtered colorless solution.
Formulation
LIFR protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPQKKGAPH DLKCVTNNLQ
VWNCSWKAPS GTGRGTDYEV CIENRSRSCY QLEKTSIKIP ALSHGDYEIT INSLHDFGSS TSKFTLNEQN VSLIPDTPEI
LNLSADFSTS TLYLKWNDRG SVFPHRSNVI WEIKVLRKES MELVKLVTHN TTLNGKDTLH HWSWASDMPL
ECAIHFVEIR CYIDNLHFSG LEEWSDWSPV KNISWIPDSQ TKVFPQDKVI LVGSDITFCC
VSQEKVLSAL IGHTNCPLIH LDGENVAIKI RNISVSASSG TNVVFTTEDN IFGTVIFAGY PPDTPQQLNC
ETHDLKEIIC SWNPGRVTAL VGPRATSYTL VESFSGKYVR LKRAEAPTNE SYQLLFQMLP NQEIYNFTLN AHNPLGRSQS TILVNITEKV YPHTPTSFKV KDINSTAVKL
SWHLPGNFAK INFLCEIEIK KSNSVQEQRN VTIKGVENSS YLVALDKLNP YTLYTFRIRC STETFWKWSK WSNKKQHLTT
EASPSKGPDT WREWSSDGKN LIIYWKPLPI NEANGKILSY NVSCSSDEET QSLSEIPDPQ HKAEIRLDKN DYIISVVAKN SVGSSPPSKI ASMEIPNDDL
KIEQVVGMGK GILLTWHYDP NMTCDYVIKW CNSSRSEPCL MDWRKVPSNS TETVIESDEF RPGIRYNFFL
YGCRNQGYQL LRSMIGYIEE LAPIVAPNFT VEDTSADSIL VKWEDIPVEE LRGFLRGYLF YFGKGERDTS KMRVLESGRS
DIKVKNITDI SQKTLRIADL QGKTSYHLVL RAYTDGGVGP EKSMYVVTKE NSHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
