Catalogue number
PRO-2216
Synonyms
Cholinergic Receptor, Nicotinic, Alpha 3 (Neuronal),
Cholinergic Receptor, Nicotinic, Alpha Polypeptide 3, NACHRA3, LNCR2, PAOD2,
Neuronal Nicotinic Acetylcholine Receptor, Alpha3 Subunit, Neuronal
Acetylcholine Receptor Subunit Alpha-3, Acetylcholine Receptor, Alpha 3
(Neuronal), Nicotinic, Neuronal acetylcholine receptor subunit alpha-3.
Introduction
Cholinergic Receptor Nicotinic Alpha 3, also known as CHRNA3 is part of
the nicotinic acetylcholine receptor family. Proteins of this family form
pentameric complexes comprised of both alpha & beta subunits. Furthermore,
CHRNA3 is ligand-gated ions channel which prticipates in neurotransmission.
CHRNA3 has been associated with an increased risk of smoking initiation as well
as an increased susceptibility to lung cancer.
Description
CHRNA3 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 232 amino acids (32-240 a.a) and
having a molecular mass of 27kDa.
CHRNA3 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CHRNA3 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSSEAEHRL
FERLFEDYNE IIRPVANVSD PVIIHFEVSM SQLVKVDEVN QIMETNLWLK QIWNDYKLKW NPSDYGGAEF
MRVPAQKIWK PDIVLYNNAV GDFQVDDKTK ALLKYTGEVT WIPPAIFKSS CKIDVTYFPF DYQNCTMKFG
SWSYDKAKID LVLIGSSMNL KDYWESGEWA IIKAPGYKHD IKYNCCEEIY PDITYSLYIR RL.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
