Catalogue number
PRO-177
Synonyms
Heterogeneous Nuclear Ribonucleoprotein C (C1/C2), HNRPC, hnRNP C1/C2, hnRNPC, C1, C2, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677.
Introduction
HNRNPC is a member of the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) which are related to pre mRNAs in the nucleus and effect pre mRNA processing and other aspects of mRNA metabolism and transport. Although all of the hnRNPs are present in the nucleus, several hnRNPs travel between the nucleus and the cytoplasm. The hnRNP proteins have specific nucleic acid binding properties.
Description
HNRNPC produced in E.Coli is a single, non-glycosylated polypeptide chain containing 313 amino acids (1-293a.a.) and having a molecular mass of 34.5kDa (Molecular weight on SDS-PAGE will appear higher).
HNRNPC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The HNRNPC protein solution (0.25mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 5mM DTT and 30% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MASNVTNKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRGKAGVKRS AAEMYGSSFD LDYDFQRDYY DRMYSYPARV PPPPPIARAV VPSKRQRVSG NTSRRGKSGF NSKSGQRGSS KSGKLKGDDL QAIKKELTQI KQKVDSLLEN LEKIEKEQSK QAVEMKNDKS EEEQSSSSVK KDETNVKMES EGGADDSAEE GDLLDDDDNE DRGDDQLELI KDDEKEAEEG EDDRDSANGE DDS.
Purity
Greater than 85% as determined by SDS-PAGE.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
