Catalogue number
ENZ-511
Synonyms
CDC34B, E2-CDC34B, UBC3B, Ubiquitin carrier protein R2, Ubiquitin-conjugating
enzyme E2-CDC34B, Ubiquitin-protein ligase R2, Ubiquitin-conjugating enzyme E2
R2, EC 6.3.2.19.
Introduction
Ubiquitin-conjugating
enzyme E2 R2 (UBE2R2) is a member of the ubiquitin-conjugating
enzyme family. Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr
protein kinase implicated in cell growth and transformation. This protein is a protein similar to the
E2 ubiquitin conjugating enzyme
UBC3/CDC34. Studies propose
that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme
functions by regulating beta-TrCP
substrate recognition and induces its
interaction with beta-TrCP, enhancing beta-catenin degradation. UBE2R2 receives ubiquitin from the E1
complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes
monoubiquitination and 'Lys-48'-linked polyubiquitination. UBE2R2 may be implicated
in degradation of katenin. Among the diseases associated with UBE2R2 are cblc,
and herpes simplex.
Description
UBE2R2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (1-238 a.a) and having a molecular mass of 29.6kDa (Molecular size on SDS-PAGE will appear higher).
UBE2R2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
UBE2R2 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQQQMT SSQKALMLEL KSLQEEPVEG FRITLVDESD LYNWEVAIFG
PPNTLYEGGY FKAHIKFPID YPYSPPTFRF LTKMWHPNIY ENGDVCISIL HPPVDDPQSG ELPSERWNPT
QNVRTILLSV ISLLNEPNTF SPANVDASVM FRKWRDSKGK DKEYAEIIRK QVSATKAEAE KDGVKVPTTL AEYCIKTKVP SNDNSSDLLY
DDLYDDDIDD EDEEEEDADC YDDDDSGNEE S
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
