Catalogue number
PKA-330
Synonyms
Phosphoinositide 3-kinase p110d/p85a, PI3Kd.
Description
The class IA PI3Kd is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3Kd in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgE induced degranulation and cytokine release. In neutrophils a role for PI3Kd in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the protein’s application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110d chain is 120.2 kDa (without Tag) and the p85a chain 83.5 kDa.
Source
Sf9 insect cells.
Physical Appearance
Sterile filtered liquid formualtion.
Formulation
PI3Kd solution in 25mM Tris-HCl, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS Page.
Unit Definition
1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute.
Specific Activity
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
