Catalogue number
ENZ-976
Synonyms
TIMP Metallopeptidase Inhibitor 4, Tissue Inhibitor Of
Metalloproteinases 4, Tissue Inhibitor Of Metalloproteinase 4, TIMP-4,
Metalloproteinase Inhibitor 4.
Introduction
Metalloproteinase inhibitor 4 (TIMP4) is a member of a family of secreted proteins which regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases. The four known members of this family are TIMP1, 2, 3 and 4. TIMP4 is produced by various tissues, particularly the brain, heart, ovary and skeletal muscle. Limited studies have shown that the TIMP4 protein has a tumor suppressive effect against Wilm92s tumor, exhibiting a negative correlation with glioma malignancy and is found in breast carcinoma cells.
Description
TIMP4 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 204 amino acids (30-224 a.a.) and
having a molecular mass of 23.5kDa (Molecular size on SDS-PAGE will appear at
approximately 18-28kDa).
TIMP4 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
TIMP4 protein solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPCSCAPAH PQQHICHSAL VIRAKISSEK VVPASADPAD TEKMLRYEIK QIKMFKGFEK VKDVQYIYTP FDSSLCGVKL EANSQKQYLL TGQVLSDGKV FIHLCNYIEP WEDLSLVQRE SLNHHYHLNC GCQITTCYTV PCTISAPNEC LWTDWLLERK LYGYQAQHYV CMKHVDGTCS WYRGHLPLRK EFVDIVQPHH HHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
