Catalogue number
ENZ-740
Synonyms
NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9, B22, LYRM3, UQOR22, Complex I-B22, CI-B22, LYR motif-containing protein 3, NADH-ubiquinone oxidoreductase B22 subunit, NDUFB9.
Introduction
NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9 (NDUFB9) which is a part of the complex I LYR family is localized to the internal mitochondrial membrane, as well as to the matrix side of the peripheral membrane. NDUFB9 serves as an accessory subunit of the multi-subunit mitochondrial membrane respiratory chain NADH dehydrogenase complex I. Complex I takes a crucial part in the transfer of electrons from NADH to the respiratory chain, a process which is vital for cellular respiration.
Description
NDUFB9 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 202 amino acids (1-179) and having a molecular mass of 24.2kDa.
NDUFB9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The NDUFB9 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAFLASG PYLTHQQKVL RLYKRALRHL ESWCVQRDKY RYFACLMRAR FEEHKNEKDM AKATQLLKEA EEEFWYRQHP QPYIFPDSPG GTSYERYDCY KVPEWCLDDW HPSEKAMYPD YFAKREQWKK LRRESWEREV KQLQEETPPG GPLTEALPPA RKEGDLPPLW WYIVTRPRER PM.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
