Catalogue number
ENZ-509
Synonyms
Nicotinamide Nucleotide Adenylyltransferase 2, C1orf15, Nicotinate-Nucleotide
Adenylyltransferase 2, NaMN Adenylyltransferase 2, NMN Adenylyltransferase 2, PNAT2,
KIAA0479, Chromosome 1 Open Reading Frame 15, Nicotinamide Mononucleotide
Adenylyltransferase 2, Pyridine Nucleotide Adenylyltransferase 2, EC 2.7.7.1, EC
2.7.7.18.
Introduction
Nicotinamide
Nucleotide Adenylyltransferase 2, also known as NMNAT2 is a member of the
nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members
of which catalyze a vital step in NAD (NADP) biosesynthetic pathway. Unlike the
other human family member, which is localized to the nucleus, and is
ubiquitously expressed; NMNAT2 is cytoplasmic, and is predominantly
expressed in the brain. Two transcript variants encoding different isoforms
have been found for NMNAT2. Among the diseases associated with NMNAT2 are
tauopathy, and systemic lupus erythematosus.
Description
NMNAT2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 327 amino acids (1-307 a.a) and having a molecular mass of 36.6kDa.
NMNAT2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
NMNAT2 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH
MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMC
QLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQN
SNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIP
GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHG
DGHVVDYLSQ PVIDYILKSQ LYINASG
Biological Activity
Specific activity: > 500 pmol/min/ug. One unit will convert 1.0 pmole of beta-NADH per minute to beta-NAD at PH 8.0 at 37°C.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
