Catalogue number
ENZ-722
Synonyms
Glutamyl-TRNA(Gln) Amidotransferase Subunit C, Glu-AdT
Subunit C, 15E1.2, Glutamyl-TRNA(Gln) Amidotransferase Subunit C Homolog
(Bacterial), Glutamyl-TRNA(Gln) Amidotransferase Subunit C Mitochondrial, Glutamyl-TRNA(Gln)
Amidotransferase Subunit C Homolog, EC 6.3.5, Protein 15E1.2.
Introduction
Glutamyl-TRNA
Amidotransferase, Subunit C also known as GATC allows the formation of properly
charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in
the mitochondria. The reaction occurs in the attendance of glutamine and ATP through
an activated gamma-phospho-Glu-tRNA(Gln). In addition, GATC is subunit of the
heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112)
and C (GATC) subunits.
Description
GATC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (1-136 a.a) and having a molecular mass of 17.5kDa (Molecular size on SDS-PAGE will appear higher).
GATC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
GATC protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD
FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA
PPGNISLPKL DEQEPFPHS
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
