Catalogue number
ENZ-933
Synonyms
Legumain,
Asparaginyl endopeptidase, Protease, cysteine 1.
Introduction
Legumain, also known as LGMN is a lysosomal cysteine protease which is found
in all mouse tissues, however it was mainly abundant in the kidney as well as
placenta. LGMN plays an essential role in the endosomal/lysosomal degradation
system as the Legumain deficiency causes the accumulation of pro cathepsins B, H
& L, another group of lysosomal cysteine proteases. Furthermore, over
expression of LGMN in tumors is important for invasion/metastasis.
Description
LGMN produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 426 amino acids (18-435a.a.) and
having a molecular mass of 48.6kDa.
(Molecular size on SDS-PAGE will appear at
approximately 40-57kDa).
LGMN is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
LGMN protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ
IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKS
GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMMNHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED
LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTIL
KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTAHDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM
DKVCLSHYLE HHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
