Catalogue number
ENZ-874
Synonyms
L-dopachrome tautomerase (EC:5.3.3.12), DCT, DT, L-dopachrome
Delta-isomerase Tyrosinase-related protein
2, TRP-2, TRP2, TYRP2.
Introduction
Dopachrome Tautomerase also known as DCT,
belongs to the tyrosinase family. DCT takes a significant part in the synthesis
of the melanin pigment including tyrosinase (Tyr), tyrosinase-related protein 1
(Tyrp1). Which are highly involved in eumelanin synthesis. The change among the
eumelanin and the pheomelanin pathways is assumed to depend on the presence of
cysteine. Hence, in the lack of cysteine, dopaquinone, the product of
tyrosinase action, is transformed to cyclodopa (leucodopachrome) and afterwards
to dopachrome (and DOPA).
Description
DCT Human Recombinant produced in Sf9 Insect cell is a
single, non-glycosylated polypeptide chain containing 455 amino acids (24-472aa.a)
and having a molecular mass of 52.1kDa (Migrates at 50-70kDa on SDS-PAGE under
reducing conditions).
DCT is fused to a 6 amino acid
His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9 Insect cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
STK3 protein solution (0.25mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
QFPRVCMTVD SLVNKECCPR
LGAESANVCG SQQGRGQCTE VRADTRPWSG PYILRNQDDR ELWPRKFFHR TCKCTGNFAG YNCGDCKFGW TGPNCERKKP PVIRQNIHSL SPQEREQFLG ALDLAKKRVH PDYVITTQHW LGLLGPNGTQ PQFANCSVYD
FFVWLHYYSV RDTLLGPGRP YRAIDFSHQG PAFVTWHRYH LLCLERDLQR LIGNESFALP YWNFATGRNE
CDVCTDQLFG AARPDDPTLI SRNSRFSSWE TVCDSLDDYN HLVTLCNGTY EGLLRRNQMG RNSMKLPTLK DIRDCLSLQK FDNPPFFQNS TFSFRNALEG FDKADGTLDS QVMSLHNLVH SFLNGTNALP HSAANDPIFV
VLHSFTDAIF DEWMKRFNPP ADAWPQELAP IGHNRMYNMV PFFPPVTNEE LFLTSDQLGY SYAIDLPVSV
EETPGWPTTH HHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
