Catalogue number
PRO-2080
Synonyms
Signal-Regulatory Protein Alpha, SHPS1, CD172
Antigen-Like Family Member A, Inhibitory Receptor SHPS-1, Macrophage Fusion
Receptor, PTPNS1, SIRP, P84, BIT, MFR, Brain-Immunoglobulin-Like Molecule With Tyrosine-Based
Activation Motifs, Brain Ig-Like Molecule With Tyrosine-Based Activation
Motifs, Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1, Tyrosine-Protein
Phosphatase Non-Receptor Type Substrate 1, Tyrosine Phosphatase SHP Substrate
1, Signal-Regulatory Protein Alpha-1 , ignal-Regulatory Protein Alpha-2, Signal-Regulatory
Protein Alpha-3, SHP Substrate 1, CD172a Antigen, Myd-1 Antigen, MyD-1 Antigen,
Sirp-Alpha-1, Sirp-Alpha-2, Sirp-Alpha-3, CD172A, SHPS-1, MYD-1, MYD1, Tyrosine-protein
phosphatase non-receptor type substrate 1.
Introduction
Signal-Regulatory Protein Alpha, SIRPA belongs to the
signal-regulatory-protein (SIRP) family, as well as the immunoglobulin super family. The members
of the SIRP family are receptor-type
transmembrane glycoproteins which are involved in the negative regulation of receptor tyrosine
kinase-coupled signaling processes. SIRPA can be phosphorylated
by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown
to recruit SH2 domain
containing tyrosine phosphatases (PTP), and perform as substrates of PTPs. SIRPA take part in signal transduction mediated by a variety
of growth factor receptors. CD47 has been shown to be a ligand for SIRPA.
Description
SIRPA Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 370 amino acids (27-373 a.a) and
having a molecular mass of 40.4kDa.
SIRPA is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
SIRPA protein solution (0.5mg/ml) containing PBS buffer
(pH 7.4), 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSGVAGEEE LQVIQPDKSV LVAAGETATL RCTATSLIPV GPIQWFRGAG
PGRELIYNQK EGHFPRVTTV SDLTKRNNMD FSIRIGNITP ADAGTYYCVK FRKGSPDDVE FKSGAGTELS
VRAKPSAPVV SGPAARATPQ HTVSFTCESH GFSPRDITLKWFKNGNELSD FQTNVDPVGE SVSYSIHSTA KVVLTREDVH SQVICEVAHV
TLQGDPLRGT ANLSETIRVP PTLEVTQQPV RAENQVNVTC QVRKFYPQRL QLTWLENGNV SRTETASTVT
ENKDGTYNWM SWLLVNVSAH RDDVKLTCQV EHDGQPAVSK SHDLKVSAHP KEQGSNTAAENTGSNERNIY.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
