Catalogue number
PRO-984
Synonyms
Ribosomal Protein S18, D6S218E, 40S Ribosomal Protein S18,
HKE3, KE-3, KE3, S18, Rhabdomyosarcoma Antigen MU-RMS-40.21, Ke-3, Ke3.
Introduction
40S ribosomal protein S18 (RPS18) is a member of the
ribosomal protein S13P family. RPS18 is located in the cytoplasm. Ribosomes, the
organelles which catalyze protein synthesis, consist of a small 40S subunit and
a large 60S subunit. Jointly these subunits are composed
of 4 RNA species and approximately 80 structurally distinct proteins. RPS18
encodes a ribosomal protein which is a component of the 40S subunit. RPS18 is a
recognized binding partner of Cofilin and has been proposed to be a novel
substrate for CaMKII. Among the diseases
associated with RPS18 are bowen-conradi syndrome, and pasteurellosis.
Description
RPS18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 175 amino acids (1-152 a.a) and having a molecular mass of 20.1kDa.
RPS18 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
RPS18 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSLVIPE KFQHILRVLN TNIDGRRKIA FAITAIKGVG RRYAHVVLRK
ADIDLTKRAG ELTEDEVERV ITIMQNPRQY KIPDWFLNRQ KDVKDGKYSQ VLANGLDNKL REDLERLKKI
RAHRGLRHFW GLRVRGQHTK TTGRRGRTVG VSKKK
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
