Catalogue number
PRO-2112
Synonyms
Y Box Binding Protein, NSEP1,DBPB, YB1, CCAAT-Binding Transcription Factor I Subunit A, Nuclease Sensitive Element Binding Protein, Enhancer Factor I Subunit A, Y-Box Transcription Factor, Y-Box-Binding Protein, DNA-Binding P, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1, EFI-A, YB-1, Major Histocompatibility Complex Class II Y Box-Binding Protein I.
Introduction
Y Box Binding Protein 1 (YBX1) is a part of the CRD-mediated complex which promotes MYC mRNA stability. YBX1 takes part in mammalian selenoprotein translation and mediates pre-mRNA alternative splicing regulation. YBX1 uses as a drug target in cancer therapy due to its ability to replicate adenovirus type 5, a commonly used vector in gene therapy.
Description
YBX1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 347 amino acids (1-324a.a) and having a molecular mass of 38.3kDa (molecular size on SDS-PAGE will appear higher). YBX1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
YBX1 protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH7.4) and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
