Catalogue number
CKI-270
Synonyms
Creatine Kinase MB Isoenzyme Type-II, CKMBITII, CKMBI, CKMB.
Introduction
CK-MB Type II possesses the naturally occurring carboxy-terminal amino acid lysine.
This occurs during a myocardial infarct (MI or heart attack) when CK-MB Type II is released from damaged heart muscle, and the C-terminal lysine is cleaved in the blood stream, thus creating CK-MB Type I. This difference can be exploited in diagnosis of an MI.
Description
CKMBITII Human Recombinant produced in Pichia Pastoris reacts with polyclonal antibodies to MB Isoenzyme in ELISA.
Source
Pichia Pastoris.
Physical Appearance
Sterile Filtered colourless liquid formulation.
Formulation
Each mg of protein contains 20mM Tris-HCl, pH-6.8, 1mM EDTA and 1mM DTT, 50% (v/v) glycerol.
Stability
CKMBITII although stable at 15°C for 7 days, should be stored below -18°C.
Please prevent freeze-thaw cycles.
Biological Activity
The enzyme activity measured by kinetic assay at 340nm was 883 IU/mg at 37 degrees Celsius, corresponding to a Specific Activity of 1,133ng/ml.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
